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Preheat oven to 350 degrees F.
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Wash and prep the squash by trimming the ends and slicing into coins. Peel and dice the onion. Place the onions and squash in a steamer basket over boiling water and steam for about 8 to 10 minutes.
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Place the squash and onions on a paper towel-lined plate and blot with additional paper towels to dry as much as possible.
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Spray a 2 1/2-quart baking dish with cooking spray and add the squash and onions. Lightly season with salt and pepper.
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In a mixing bowl, mix together the soup, sour cream, and eggs. Pour the mixture over the squash and onions in the baking dish and stir to combine well. Place the American cheese slices on top of the mixture.
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Add the stuffing mix to a mixing bowl. Melt the butter in the microwave and pour over the stuffing mix. Mix together well, being sure to get melted butter on all the stuffing mix.
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Evenly distribute the stuffing mix and butter over the cheese slices.
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Cover the dish with aluminum foil and bake for approximately 20 to 25 minutes. Uncover and bake for an additional 10 minutes or so or until the stuffing mix is browned.
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Serve